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human acute monocytic leukemia cell line thp1 (ATCC)

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ATCC human acute monocytic leukemia cell line thp1
Human Acute Monocytic Leukemia Cell Line Thp1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 20139 article reviews

human acute monocytic leukemia cell line thp1 - by Bioz Stars, 2026-09

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Article Title: Identification of a novel transcript of human MD2 gene.
Article Snippet: Myeloid differentiation protein 2 (MD2) regulates bacterial lipopolysaccharide (LPS) triggered anti-bacterial immune response as a broker between LPS and Toll-like receptor 4 (TLR4).. In this study, we identified a novel naturally occurring spliceosome of human MD2, termed as MD2-T3.. This transcript lacked two exons compared with MD2-T1.

Multiple Displacement Amplification:

Article Title: XIAOPI Formula Inhibits Breast Cancer Stem Cells via Suppressing Tumor-Associated Macrophages/C-X-C Motif Chemokine Ligand 1 Pathway
Article Snippet: .. The non-malignant human mammary epithelial cell line MCF-10A, human breast cancer cell line MDA-MB-231, mouse breast cancer cell line 4T1, human acute monocytic leukemia cell line Thp1 and mouse macrophage cell line Raw264.7 were obtained from the American Type Culture Collection. ..

Article Title: XIAOPI Formula Inhibits Breast Cancer Stem Cells via Suppressing Tumor-Associated Macrophages/C-X-C Motif Chemokine Ligand 1 Pathway.
Article Snippet: .. The non-malignant human mammary epithelial cell line MCF10A, human breast cancer cell line MDA-MB-231, mouse breast cancer cell line 4T1, human acute monocytic leukemia cell line Thp1 and mouse macrophage cell line Raw264.7 were obtained from the American Type Culture Collection. ..

Article Title: Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling
Article Snippet: Mouse TNBC 4 T1 cells (KG338) and Raw264.7 macrophages (KG240) were obtained from Nanjing KeyGen Biotech (Nanjing, China). .. Human acute monocytic leukemia cell line THP1 and TNBC cell line MDA-MB-231 were obtained from the American Type Culture Collection. .. 100 ng/ml phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, Missouri, USA) was used to induce attachment and differentiation of THP1 monocytes into macrophages.

Article Title: Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling.
Article Snippet: Mouse TNBC 4 T1 cells (KG338) and Raw264.7 macrophages (KG240) were obtained from Nanjing KeyGen Biotech (Nanjing, China). .. Human acute monocytic leukemia cell line THP1 and TNBC cell line MDA-MB-231 were obtained from the American Type Culture Collection. .. 100 ng/ml phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, Missouri, USA) was used to induce attachment and differentiation of THP1 monocytes into macrophages.

Cell Culture:

Article Title: Antibodies and derivatives thereof
Article Snippet: Data obtained were expressed as % of response and analyzed with GraphPad Prism 5 software using a nonlinear regression analysis, log(inhibitor) vs. normalized response—variable slope equation. .. Human acute monocytic leukemia cell line THP1 (ATCC) was cultured in RPMI1640/GLUTAMAX (Invitrogen)+10% Foetal Bovine Serum (Invitrogen)+Pen/Strep. and maintained between 2-9×100,000 cells/ml. ..

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( A and B ) Volcano plot of differentially expressed lncRNAs in T2D monocytes versus controls ( A ) and their genomic classification ( B ). ( C ) Schematic showing genomic organization of lncRNA DRAIR and nearby CPEB2 gene, along with RNA-seq tracks from CD14 + monocytes from T2D and control subjects. DRAIR and CPEB2 show downregulation in T2D monocytes versus controls (log 2 fold = –1.75, FDR = 0.029, and log 2 fold = –2.19, FDR = 1.18 x 10 –5 , respectively; n = 5 each). H3K4me3 track from CD14 + monocytes (from ENCODE) is shown to indicate shared promoter region for both genes. Map not drawn to scale. ( D and E ) qPCR validation of DRAIR and CPEB2 downregulation in T2D monocytes versus control (* P < 0.05, by t tests, n = 6). ( F – H ) qPCR results showing downregulation of DRAIR in primary human CD14 + monocytes ( F ), primary human macrophages ( G ), and THP1 monocytes ( H ) treated with normal glucose (NG, 5.5 mM), high glucose (HG, 25 mM, 72 hours), palmitic acid (PA, 200 μM, 24 hours), and HG + PA (HP) and IL-1β (10 ng/mL). * P < 0.05 and ** P < 0.01, as determined by 1-way ANOVA and Dunnett’s multiple-comparison test in F and H ( n = 3–6) and t test in G ( n = 3).

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A and B ) Volcano plot of differentially expressed lncRNAs in T2D monocytes versus controls ( A ) and their genomic classification ( B ). ( C ) Schematic showing genomic organization of lncRNA DRAIR and nearby CPEB2 gene, along with RNA-seq tracks from CD14 + monocytes from T2D and control subjects. DRAIR and CPEB2 show downregulation in T2D monocytes versus controls (log 2 fold = –1.75, FDR = 0.029, and log 2 fold = –2.19, FDR = 1.18 x 10 –5 , respectively; n = 5 each). H3K4me3 track from CD14 + monocytes (from ENCODE) is shown to indicate shared promoter region for both genes. Map not drawn to scale. ( D and E ) qPCR validation of DRAIR and CPEB2 downregulation in T2D monocytes versus control (* P < 0.05, by t tests, n = 6). ( F – H ) qPCR results showing downregulation of DRAIR in primary human CD14 + monocytes ( F ), primary human macrophages ( G ), and THP1 monocytes ( H ) treated with normal glucose (NG, 5.5 mM), high glucose (HG, 25 mM, 72 hours), palmitic acid (PA, 200 μM, 24 hours), and HG + PA (HP) and IL-1β (10 ng/mL). * P < 0.05 and ** P < 0.01, as determined by 1-way ANOVA and Dunnett’s multiple-comparison test in F and H ( n = 3–6) and t test in G ( n = 3).

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: RNA Sequencing, Control, Biomarker Discovery, Comparison

( A – H ) Effects of DRAIR overexpression. qPCR analysis of indicated genes in THP1 cells transduced with lentivruses expressing DRAIR or a control vector (EV). * P < 0.05; ** P < 0.01; *** P < 0.001, by t test ( n = 3). ( I – L ) Effects of DRAIR knockdown with siRNAs. THP1 cells were transfected with control siRNA (siNC) or siRNA targeting DRAIR (siDR). Two days later, cells were treated with or without with LPS (100 ng/mL) for 24 hours, and gene expression was analyzed by qPCR. Results expressed as fold over siNC control. ( M – O ) qPCR analysis of THP1 cells transfected with siNC or siDR treated ± PMA (20 ng/mL) for 24 hours. Results expressed as fold over siNC control (Ctrl). For I – O , * P < 0.05; *** P < 0.001 versus siNC control and † P < 0.05 versus siNC LPS as determined by 2-way ANOVA (I–L), 1-way ANOVA (M–O), and Sidak’s multiple-comparison test ( n = 3). ( P and Q ) THP1 cells transfected with siDR or siNC were treated ± TNF-α (10 ng/mL, 3 hours), labeled with DAPI, and used in monocyte–endothelial cell (monocyte-EC) adhesion assays. Images of bound monocytes were collected using a fluorescence microscope ( P ). Total original magnification, ×100. Bound monocytes from multiple wells/group were quantified using ImageJ software ( Q ). * P < 0.05; *** P < 0.001 as determined by 1-way ANOVA and Sidak’s multiple-comparison test (versus siNC control, n = 25–29). ( R ) Phagocytosis assays were performed with fluorescently labeled E . coli bioparticles in THP1 macrophages transfected with siDR or siNC and treated with IL-4 (20 ng/mL) or LPS (100 ng/mL) for 24 hours. Results shown as fluorescence from phagocytosed particles. ** P < 0.01; *** P < 0.001 as determined by multiple unpaired t tests, with correction for multiple comparisons using the Holm-Sidak method (versus siNC, n = 6).

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A – H ) Effects of DRAIR overexpression. qPCR analysis of indicated genes in THP1 cells transduced with lentivruses expressing DRAIR or a control vector (EV). * P < 0.05; ** P < 0.01; *** P < 0.001, by t test ( n = 3). ( I – L ) Effects of DRAIR knockdown with siRNAs. THP1 cells were transfected with control siRNA (siNC) or siRNA targeting DRAIR (siDR). Two days later, cells were treated with or without with LPS (100 ng/mL) for 24 hours, and gene expression was analyzed by qPCR. Results expressed as fold over siNC control. ( M – O ) qPCR analysis of THP1 cells transfected with siNC or siDR treated ± PMA (20 ng/mL) for 24 hours. Results expressed as fold over siNC control (Ctrl). For I – O , * P < 0.05; *** P < 0.001 versus siNC control and † P < 0.05 versus siNC LPS as determined by 2-way ANOVA (I–L), 1-way ANOVA (M–O), and Sidak’s multiple-comparison test ( n = 3). ( P and Q ) THP1 cells transfected with siDR or siNC were treated ± TNF-α (10 ng/mL, 3 hours), labeled with DAPI, and used in monocyte–endothelial cell (monocyte-EC) adhesion assays. Images of bound monocytes were collected using a fluorescence microscope ( P ). Total original magnification, ×100. Bound monocytes from multiple wells/group were quantified using ImageJ software ( Q ). * P < 0.05; *** P < 0.001 as determined by 1-way ANOVA and Sidak’s multiple-comparison test (versus siNC control, n = 25–29). ( R ) Phagocytosis assays were performed with fluorescently labeled E . coli bioparticles in THP1 macrophages transfected with siDR or siNC and treated with IL-4 (20 ng/mL) or LPS (100 ng/mL) for 24 hours. Results shown as fluorescence from phagocytosed particles. ** P < 0.01; *** P < 0.001 as determined by multiple unpaired t tests, with correction for multiple comparisons using the Holm-Sidak method (versus siNC, n = 6).

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: Over Expression, Transduction, Expressing, Control, Plasmid Preparation, Knockdown, Transfection, Gene Expression, Comparison, Labeling, Fluorescence, Microscopy, Software

( A – D ) qPCR analysis of indicated genes in THP1 monocytes (THP1) before and after differentiation into macrophages (TMac) with PMA (20 ng/mL) for 24 hours. * P < 0.05; **** P < 0.0001 as determined by unpaired t test ( n = 3). ( E – H ) Expression of indicated genes in THP1 macrophages treated with IL-4 or IL-13 or a combination of both (20 ng/mL each). * P < 0.05 ( n = 3) as determined by 1-way ANOVA and Dunnett’s multiple comparisons tests. ( I – K ) Gene expression analysis in THP1 cells transiently transfected with control pcDNA3.1 (pCD) or KLF4 expression (pKLF4) plasmids. Gene expression analyses were performed 48 hours after transfection. ** P < 0.01; *** P < 0.001 versus pCD as determined by unpaired t test ( n = 3). ( L ) ChIP-qPCR analysis of ChIP assays with KLF4 antibody with indicated promoter primers (** P < 0.01 versus PPIA promoter, n = 3). ( M ) Schematic of the reporter plasmid (pDRluc) with DRAIR promoter cloned upstream of firefly luciferase reporter gene. KLF4 site (–760) in DRAIR promoter (not to scale). ( N – P ) Luciferase activities with THP1 cells cotransfected with pDRluc and internal control Renilla luciferase. In addition, plasmids pKLF4 and pCDNA3.1 were also cotransfected in P . One day after transfection, cells were treated as indicated for 24 hours. Luciferase activities are reported as fold over controls. In N , Ctrl, control; PA, palmitic acid (200 μM); IL-4, 20 ng/mL; in O , PMA-PMA 20 ng/mL. * P < 0.05, ** P < 0.01 as determined by 1-way ANOVA followed by Dunnett’s multiple-comparison test ( N and P , n = 10–13) and unpaired t test for O ( n = 5).

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A – D ) qPCR analysis of indicated genes in THP1 monocytes (THP1) before and after differentiation into macrophages (TMac) with PMA (20 ng/mL) for 24 hours. * P < 0.05; **** P < 0.0001 as determined by unpaired t test ( n = 3). ( E – H ) Expression of indicated genes in THP1 macrophages treated with IL-4 or IL-13 or a combination of both (20 ng/mL each). * P < 0.05 ( n = 3) as determined by 1-way ANOVA and Dunnett’s multiple comparisons tests. ( I – K ) Gene expression analysis in THP1 cells transiently transfected with control pcDNA3.1 (pCD) or KLF4 expression (pKLF4) plasmids. Gene expression analyses were performed 48 hours after transfection. ** P < 0.01; *** P < 0.001 versus pCD as determined by unpaired t test ( n = 3). ( L ) ChIP-qPCR analysis of ChIP assays with KLF4 antibody with indicated promoter primers (** P < 0.01 versus PPIA promoter, n = 3). ( M ) Schematic of the reporter plasmid (pDRluc) with DRAIR promoter cloned upstream of firefly luciferase reporter gene. KLF4 site (–760) in DRAIR promoter (not to scale). ( N – P ) Luciferase activities with THP1 cells cotransfected with pDRluc and internal control Renilla luciferase. In addition, plasmids pKLF4 and pCDNA3.1 were also cotransfected in P . One day after transfection, cells were treated as indicated for 24 hours. Luciferase activities are reported as fold over controls. In N , Ctrl, control; PA, palmitic acid (200 μM); IL-4, 20 ng/mL; in O , PMA-PMA 20 ng/mL. * P < 0.05, ** P < 0.01 as determined by 1-way ANOVA followed by Dunnett’s multiple-comparison test ( N and P , n = 10–13) and unpaired t test for O ( n = 5).

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: Expressing, Gene Expression, Transfection, Control, ChIP-qPCR, Plasmid Preparation, Clone Assay, Luciferase, Comparison

( A – D ) Expression of indicated genes was analyzed by qPCR in THP1 cells transfected with siNC and siRNA targeting CPEB2 (siCPEB2). * P < 0.05; *** P < 0.001 ( n = 5) versus untreated siNC as determined by unpaired t test. ( E and F ) Images and quantification of monocyte–endothelial cell (monocyte-EC) adhesion assays. THP1 monocytes transfected with siCPEB2 or siNC were treated ± TNF-α (10 ng/mL, 3 hours), fluorescently labeled with DAPI, and incubated with EC plated in 24-well plates. EC monolayers were washed with PBS, and images were collected using a fluorescence microscope. Total original magnification, ×100. Bound monocytes (blue color spots) were counted using ImageJ software. ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus untreated siNC and versus untreated siCPEB2 as determined by 1-way ANOVA and Sidak’s multiple-comparison test ( n = 16–19).

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A – D ) Expression of indicated genes was analyzed by qPCR in THP1 cells transfected with siNC and siRNA targeting CPEB2 (siCPEB2). * P < 0.05; *** P < 0.001 ( n = 5) versus untreated siNC as determined by unpaired t test. ( E and F ) Images and quantification of monocyte–endothelial cell (monocyte-EC) adhesion assays. THP1 monocytes transfected with siCPEB2 or siNC were treated ± TNF-α (10 ng/mL, 3 hours), fluorescently labeled with DAPI, and incubated with EC plated in 24-well plates. EC monolayers were washed with PBS, and images were collected using a fluorescence microscope. Total original magnification, ×100. Bound monocytes (blue color spots) were counted using ImageJ software. ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus untreated siNC and versus untreated siCPEB2 as determined by 1-way ANOVA and Sidak’s multiple-comparison test ( n = 16–19).

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: Expressing, Transfection, Labeling, Incubation, Fluorescence, Microscopy, Software, Comparison

( A ) qPCR analysis of RNA from ChIRP assays with indicated primers. Percentage of input values from 2 experiments were log transformed. ChIRP assays were performed using biotinylated DRAIR antisense oligonucleotides in THP1 cells. RNA-recovered from ChIRP assays was analyzed by qPCR and DNA by DNA sequencing (ChIRP-seq). ( B ) Genomic distribution of DRAIR binding sites (Dbs) identified from ChIRP-seq analysis. ( C ) GO terms enriched in genes nearby (±250 kb) Dbs as determined by IPA. ( D and E ). Schematic of Dbs in the intronic region of OPTC gene (OPTC-Dbs) ( D ) and its potential interaction with upstream region of CHIT1 gene ( E ) identified using Hi-C plotter (CHiCP) tool. ChIRP1 and ChIRP2 in B and D refer to duplicates. ( F ) ChIRP-qPCR analysis of ChIRP-DNA using primers for indicated DRAIR binding sites. THP1 cell lysates were treated ± pancreatic ribonuclease A (RNase A), and ChIRP assays were performed with biotinylated DRAIR probes. RNase A–treated samples served as negative controls. Percentage of input values from 2 experiments were log transformed. ( G – I ) qPCR analysis of indicated genes in THP1 cells overexpressing DRAIR versus control vector (EV). * P < 0.05; **** P < 0.0001 versus EV as determined by unpaired t test ( n = 3).

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A ) qPCR analysis of RNA from ChIRP assays with indicated primers. Percentage of input values from 2 experiments were log transformed. ChIRP assays were performed using biotinylated DRAIR antisense oligonucleotides in THP1 cells. RNA-recovered from ChIRP assays was analyzed by qPCR and DNA by DNA sequencing (ChIRP-seq). ( B ) Genomic distribution of DRAIR binding sites (Dbs) identified from ChIRP-seq analysis. ( C ) GO terms enriched in genes nearby (±250 kb) Dbs as determined by IPA. ( D and E ). Schematic of Dbs in the intronic region of OPTC gene (OPTC-Dbs) ( D ) and its potential interaction with upstream region of CHIT1 gene ( E ) identified using Hi-C plotter (CHiCP) tool. ChIRP1 and ChIRP2 in B and D refer to duplicates. ( F ) ChIRP-qPCR analysis of ChIRP-DNA using primers for indicated DRAIR binding sites. THP1 cell lysates were treated ± pancreatic ribonuclease A (RNase A), and ChIRP assays were performed with biotinylated DRAIR probes. RNase A–treated samples served as negative controls. Percentage of input values from 2 experiments were log transformed. ( G – I ) qPCR analysis of indicated genes in THP1 cells overexpressing DRAIR versus control vector (EV). * P < 0.05; **** P < 0.0001 versus EV as determined by unpaired t test ( n = 3).

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: Transformation Assay, DNA Sequencing, Binding Assay, Hi-C, Control, Plasmid Preparation

$( A ) qPCR of RNA recovered from ChIRP with DRAIR probes or negative control luciferase (LUC) probes. Cell lysates from THP1 monocytes overexpressing DRAIR were subjected to ChIRP assays with biotinylated DRAIR probes and negative control biotinylated LUC probes. ChIRP complexes were captured on streptavidin beads, and RNA from an aliquot of beads was analyzed by qPCR with DRAIR primers. ( B ) The nucleic acid–protein complexes from ChIRP were fractionated on SDS-PAGE and subjected to mass spectrometry to identify proteins interacting with DRAIR probes. STRING analysis of indicated DRAIR -interacting proteins identified by ChIRP mass spectrometry . Colors represent GO biological functions shown in . ( C ) Immunoblotting of proteins from RNA pulldown assays using DRAIR and DRAIR antisense (DRAIR-AS) probes with G9a antibody. ( D ) qPCR analysis of RNA recovered after RNA IP with indicated antibodies. ( E – M ) ChIP-qPCR analysis of DNA recovered from ChIP assays using lysates from THP1 cells overexpressing DRAIR and empty vector pcDNA3.1 (EV) with antibodies against H3K9me2 ( E – H ), G9a ( I – L ), and H3K27me3 ( M ) using indicated gene promoter primers. ( N and O ) Immunoblotting of THP1 nuclear extracts with indicated antibodies ( N ) and quantification of G9a in nuclear extracts ( O ). THP1 cells were treated with NG (5.5 mM glucose) and HG (25 mM glucose) for 72 hours. Palmitic acid (PA, 200 μM) was also added in the final 24 hours to NG- (PA) and HG-treated (HP) cells. * P < 0.05; ** P < 0.01; *** P < 0.001 ( n = 3) as determined by unpaired t test ( E – L ) and 2-way ANOVA with Sidak’s ( M ), and 1-way ANOVA with Dunnett’s ( O ) multiple-comparison testing.$

Journal: JCI Insight

Article Title: lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms

doi: 10.1172/jci.insight.143289

Figure Lengend Snippet: ( A ) qPCR of RNA recovered from ChIRP with DRAIR probes or negative control luciferase (LUC) probes. Cell lysates from THP1 monocytes overexpressing DRAIR were subjected to ChIRP assays with biotinylated DRAIR probes and negative control biotinylated LUC probes. ChIRP complexes were captured on streptavidin beads, and RNA from an aliquot of beads was analyzed by qPCR with DRAIR primers. ( B ) The nucleic acid–protein complexes from ChIRP were fractionated on SDS-PAGE and subjected to mass spectrometry to identify proteins interacting with DRAIR probes. STRING analysis of indicated DRAIR -interacting proteins identified by ChIRP mass spectrometry . Colors represent GO biological functions shown in . ( C ) Immunoblotting of proteins from RNA pulldown assays using DRAIR and DRAIR antisense (DRAIR-AS) probes with G9a antibody. ( D ) qPCR analysis of RNA recovered after RNA IP with indicated antibodies. ( E – M ) ChIP-qPCR analysis of DNA recovered from ChIP assays using lysates from THP1 cells overexpressing DRAIR and empty vector pcDNA3.1 (EV) with antibodies against H3K9me2 ( E – H ), G9a ( I – L ), and H3K27me3 ( M ) using indicated gene promoter primers. ( N and O ) Immunoblotting of THP1 nuclear extracts with indicated antibodies ( N ) and quantification of G9a in nuclear extracts ( O ). THP1 cells were treated with NG (5.5 mM glucose) and HG (25 mM glucose) for 72 hours. Palmitic acid (PA, 200 μM) was also added in the final 24 hours to NG- (PA) and HG-treated (HP) cells. * P < 0.05; ** P < 0.01; *** P < 0.001 ( n = 3) as determined by unpaired t test ( E – L ) and 2-way ANOVA with Sidak’s ( M ), and 1-way ANOVA with Dunnett’s ( O ) multiple-comparison testing.

Article Snippet: Human THP1 monocytic cell line (American Type Culture Collection [ATCC]) was used to characterize DRAIR functions and mechanisms of actions.

Techniques: Negative Control, Luciferase, SDS Page, Mass Spectrometry, Western Blot, ChIP-qPCR, Plasmid Preparation, Comparison

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human acute monocytic leukemia cell line thp1 (ATCC)

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